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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 18. Phase-micrograph time series of video-captured images showing the sperm decondensation and pronuclear formation. Sperm nu- cleus (arrowhead) was visible in the egg cortex by 4 mm PI. (B) Sperm chromatin (arrowhead) decondensation occurred by 10 min PI. (C) Sperm pronuclear (arrowhead) formation began by 30 min PI. Similar results were observed in eggs incubated in 100 iM colchicine prior to insemination (D-G)orin 12
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