HPLC has become a backbone of synthetic and analytical chemistry, meaning any serious lab has at least one HPLC system. Due to the broad range of different compounds and solvents everyday chemists ...

High performance liquid chromatography (HPLC) is an analytical technique used to separate, identify, quantify, and purify individual components of a mixture. This technique is very common in ...

For some chromatographers, their first inkling that high-performance liquid chromatography was going to be a big deal came in 1969. That year, they traveled to Las Vegas to attend the fifth in a ...

When conducting reproducible and interference-free analysis by liquid chromatography, the purity of the solvents used, particularly the purity of water, is crucial for the sensitivity of this method, ...

As analytical technology continues to advance, both regulatory agencies and pharmaceutical companies are keen to avail themselves of these enhancements. In the case of chromatography, evolution from ...

High performance liquid chromatography (HPLC) was first described in a landmark paper by Csaba Horvath and Seymour Lipsky in 1966, followed by the 1967 debut of the Waters ALC-100. Over the next ...

Operating HPLC at higher than ambient temperatures can improve peak shapes and enable faster run times. Preheating the mobile phase is significant in high temperature liquid chromatography as the ...

The use of acidic MPAs (pH 2–4) is the default in pharmaceutical testing because they suppress silanol ionization (from Si-OH to Si-O -) and improve peak shape for basic drugs. However, highly ...

Liquid chromatography is a technique of key importance for many areas of chemical analysis and one that has evolved with the progression of technology and instrumentation over time. Here we highlight ...

High performance liquid chromatography, more commonly known by the acronym HPLC, is a method used to identify substances in a mixture. There are several types of HPLC, such as reversed-phase, ...